Optimization of extraction using surface response methodology and quantification of cannabinoids in female inflorescences of marijuana (<i>Cannabis sativa</i> L.) at three altitudinal floors of Peru

Abstract The aim of this study was to extract and quantify cannabinoids from female inflorescences Cannabis sativa L. three altitudinal floors Peru, by optimizing the amplitude, time, methanol concentration in ultrasound-assisted extraction required maximize cannabidiol (CBD), delta-9-tetrahydrocannabinol (Δ9-THC) content, yields. Optimal conditions were determined response surface central composite design used. quadratic model adequate for yield, Δ9-THC, CBD with R 2 values 0.998, 0.985, 0.991 respectively. 99% radiation 20 min 96% ethanol concentration. optimized C. had a yield 24.12%, 0.62% CBD, 5.973% THC. content studied Junín Region at altitudes between 2,070 3,274 m above sea level (m asl) 0.1 0.4%, THC 2.2 6%, 10–24%; Ayacucho region an altitude 2,627 asl 0.62 0.65%, 6.21–6.72%, 23.8–24%; Huánuco 660–711 it 0.55 8.11 8.92%, 24.3 29.7%. It concluded present work that parameters such as solvent directly influence same way influences cannabinoids, being lower yields higher planting altitude.